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How Do You Calculate Concentration From Internal Standards? [Solved]

A linear equation (y=mx + b) is obtained. Concentration of the analyte is calculated by solving for x when y is the ratio of analyte signal/internal standard signal in the unknown sample. Either one standard or several standards, to create a calibration curve, may be used.2 Oct 2020

Internal standards

Corrections: At 2 minutes 35 seconds, I accidentally moved the wrong math over. It should be F = (20000/20)/(8000/10) …

Internal standard

So that’s how to use an

Internal Standards

The method of